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Description

Isopropyl--D-thiogalactopyranoside (IPTG) was added to the final concentration of 0.4 mM to induce the expression of recombinant protein at 25C for at least 5 h

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The activity of GDH is inhibited by ADP-ribosylation of the C172 residue at the active site of GDH

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doi: 10.1146/annurev-immunol-032712-100003 98 SrinivasanVMaestroniGJCardinaliDPEsquifinoAIPerumalSRMillerSC

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