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doi: 10.1016/j.molcel.2012.09.025 19

The pH during the reaction is low, which may cause chromogenic inhibition

Liz graduated from Pepperdine University with a degree in Biology in 1994

Nature neuroscience 2019;22, 18061819, doi: Schwab, N

Cys is the preferable amino acid substrate that is -glutamylated in the -GCS reaction (the Michaelis constant (Km) = 0.10.3 mM), but Cys-mimetic 2-aminobutyric acid (2AB) is also a good substrate [3]

This specific property has become the focus of numerous research strategies, such as the development of senolytic agents like FOXO4-DRI, which selectively disrupt the FOXO4p53 interaction, thereby enabling the targeted elimination of dysfunctional cells