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The mechanism underlying heart rate reduction can be attributed to the acetylcholine released by vagal parasympathetic fibers (Loffelholz and Pappano 1985) that innervate the sinoatrial and the atrioventricular nodes, with negative chronotropic and dromotropic effects, respectively (Coote 2013

The process usually looks like this

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5-Amino-1MQ is orally bioavailable with an estimated half-life of 24 hours based on murine pharmacokinetic modeling, requiring once or twice daily dosing to maintain NNMT inhibition

It has shown activity at very low doses (nanogram to microgram per kilogram) without demonstrable toxicity in animal studies [11]

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