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"Androgen receptor specifically interacts with a novel p21-activated kinase, PAK6"

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Inhibition of -glutamyl transpeptidase (GGT) impaired the ability of GSH or cell-permeable GSH to restore mTORC1 signaling and the ISR, suggesting that the capacity of GSH to release cysteine, but not GSH per se, regulated the signaling networks

The suspension was incubated for 1 h at room temperature

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However, these assays do require acid-ethanol precipitation or addition of excess IGF-2 to minimize binding protein interference (Guha et al., 2013)