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Due to their high efficacy as novel performance-enhancing substances, detection of GHRPs in biological matrix has become a focus in anti-doping analysis

2.5 Pc GKB4 mycelial protein extraction Mycelia were harvested by vacuum filtration from six bulk cultures (400 mL RMM broth in 2 L conical flasks) of both untreated controls (no Phi) and Phi-treated (30 g/mL) cultures separately, grown for 6 dpi at 22C in the dark with shaking at 150 rpm, lyophilized and stored at 80C until further use

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