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Structural reorganization of of teractions in stabilization oy NMR and REMD simulations

As long as storage temperature remains below this glass transition point, the peptide molecules stay locked in place, unable to undergo the chemical reactions that would degrade them

Arch Gen Psychiatry 57 : 11391147

M.Opoku-AgyemanM.DomfehO.et al

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Samples were washed and subsequently incubated with secondary antibody for 45 min at RT (Table 3) Sections were counterstained either with the diaminobenzidine substrate kit (Nacalai, USA) followed by hematoxylin, or sections were stained with 6-diamidino-2-phenylindole (DAPI, Thermo Fisher, USA) for visualization of cell nuclei as previously described