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Given that insulin signaling activates PI3K-AKT to promote adipogenesis, in part by upregulating adipogenic transcription factors such as PPAR 38,39 , our data suggest that reduced AKT activation underlies the impaired differentiation phenotype observed in GPR146-deficient cells

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K-means clustering of amplicon sequence variant data confirmed that 5-Amino-1MQ treatment generated a distinct cecal microbiome profile from all other treatment groups including diet-switched vehicle controls indicating that NNMT inhibition produces microbiome changes that are separable from those produced by dietary composition alone

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