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2.1.2 Echocardiography Cardiac echocardiography was performed on day 21 in mice anesthetized with 2% isoflurane gas and probed by an ultra-high resolution small animal ultrasound imaging system (SigmaVET, Esaote, Italy)

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Following sublingual administration, Drug A is 100 % bioavailable while following rectal administration, Drug A bioavailability is between 40 and 100 %, depending how far up the rectum the drug product is inserted Measurement of Bioavailability For intravenous injection, the whole of drug dose is delivered directly into the systemic circulation, thus it will always have 100 % bioavailability (F = 1) and the maximal plasma concentration (C max ) is reached instantaneously (T max = 0 min)

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Glutathione Synthesis: Supports the bodys natural production and recycling of glutathione