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For the flow cytometry assay, 3 10 4 cells/well/40 L were incubated with 5 nM of mono- or bispecific antibody constructs in flow buffer (DPBS w/o Ca and Mg (PAN-Biotech) with 3% heat inactivated FBS (Gibco)) for 1 h at 4 C in a V-Bottom 384 well plate (Greiner)

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