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Briefly, cells were suspended in sucrose-mannitol buffer (20 mM HEPES, pH 7.5, 2 mM EDTA, 70 mM sucrose, 220 mM mannitol, 5 mM NaF, protease inhibitor cocktail [Roche]) and homogenized using a Teflon homogenizer

Questultimo un tripeptide naturale contenente zolfo, formato dagli aminoacidi L-glicina, acido glutammico e L-cisteina

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315 A novel iron chelator, FerroTerminator1(FOT1), has demonstrated potent iron scavenging capacity and a good safety profile, effectively reversing liver injury in multiple MASH models without notable toxic side effects

Proximity ligation assay (PLA) for Cntfr and Syt1 Labelling of Cntfr was performed as described above also including mouse anti-Syt1 luminal antibodies (1:150 dilution, Synaptic Systems, cat