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Highlights from this retrospective review of 71 patients with PHG in India include a statistically significant association with B12 deficiency in the PHG population compared with the control population, along with statistically higher TSH levels

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Patients were stratified into high- and low-risk groups based on the optimal cutoff value of the choline metabolism-related risk score to assess the prognostic accuracy of the choline metabolism-related signature (222)

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Single-particle structure determination used established protocols in RELION for image classification and gold-standard refinement to prevent over-fitting 44

Sections were incubated with Alexa Fluor goat anti-rabbit IgG and Alexa Fluor goat anti-mouse IgG (1:500, Life Technologies, Grand Island, NY) at RT for 1 h and counterstained with DAPI