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Monitor solution clarity before each injection preparation activity

Statistical analysis revealed a slight decrease in pollen viability in snx1 / vps29 +/ compared to WT, indicating that the SNX1/VPS29 mutation had a minor effect on pollen viability (Supplementary Fig

Your provider may adjust the protocol based on your results

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of bacteria inoculated into the bladders, then humanely euthanized at 6 h post-infection, and bacterial burdens were determined to assess acute outcomes