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GHK-Cu also stimulates both collagen synthesis and matrix metalloproteinases that break collagen down, so unbalanced or excessive use could in principle disrupt extracellular-matrix homeostasis

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1-1.5 mg of nuclear lysates were diluted in IP buffer, pre-cleared with 15 l A/G plus agarose beads (Santa Cruz) and incubated for 5 hours with 20 l of GFP-Trap beads (Proteintech)

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Mn uptake via voltage-gated calcium channels in dopaminergic neurons has also been reported (Dui et al., 2013), but again at high Mn concentrations