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However, bearing in mind that the gliA promoter lacks of an obvious GliZ binding motif, the correlation between gliZ expression and gliotoxin biosynthesis, the essential role of ZafA for gliA expression during zinc starvation and the close proximity of the ZafA and GipA binding sites in the gliA promoter, it would appear more feasible that GipA, whose activity could also be influenced by the intracellular amount of gliotoxin, plays a role as a modulator of the ZafA-mediated induction of gliA under zinc starvation to adjust the gliA expression level to the intracellular amount of gliotoxin

But also I just want to, before I come to that, I just wanted to say that it was really insightful to listen to what you were saying, Robert