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The DAB images (brown in color) were calibrated using area fraction ( Statistical analysis The data were statistically analyzed using IBM SPSS, version 26.0

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Coverslips were washed, mounted onto microscope slides using ProLong Diamond Antifade Mountant with DAPI (P36966, Thermo Fisher Scientific) and imaged with a 63X oil objective Leica Stellaris 8 inverted confocal microscope from the FILM facility at Imperial College London

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