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(2) treatment with buthionine sulphoximine (BSO, 1000 M) to block de novo GSH synthesis for 2448 h

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Nasal delivery avoids injection site risks (infection, bruising, irritation) but introduces nasal mucosal irritation and formulation stability concerns

Methods Expression and purification of recombinant OsDHAR Recombinant OsDHAR was expressed in Escherichia coli NiCo21(DE3), purified and crystallized as described previously 30

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