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To generate both scExe4 and Cas9 plasmid-loaded LNPs, the molecular ratio of DLin-MC3-DMA/Cholesterol/DSPC/PEG2000-DMG was 50/38.5/10/1.5, following a previously established protocol 23,24,25

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doi: 10.1016/j.ejphar.2010.10.008 91 IwaiT.SawabeT.TanimitsuK.SuzukiM.Sasaki-HamadaS.OkaJ

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