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Next, the cells were suspended in 95 l of Cell Line Nucleofector Solution V (Lonza), and 5 l of 20 M GLP-1R siRNA (siGLP-1R 4 or siGLP-1R 5) or siControl was added

Clinical trial registration Not applicable

A new commentary in the Journal of Managed Care & Specialty Pharmacy makes the distinction explicit

Bohmer, T., Boen, A., and Hoymork, S

Moreover, using mutant GLP-1Rs, Ge et al (26) described that even with a mutation of the putative signal sequence, ligand binding to the mutant receptor was observed with conserved cAMP production

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