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The results suggested that TB-500 not only potentially enhanced the viability, angiogenesis, and migratory ability of HUVEC but possibly also promoted the expression of angiopoietin-2 (Ang2), TEK receptor tyrosine kinase 2 (tie2), vascular endothelial growth factor A (VEGFA), NOTCH1 intracellular domain (N1ICD), Notch receptor 3 (Notch3), NF-B, and phosphorylated (p)-p65 in HUVEC

It also suppressed pro-inflammatory cytokines (e.g., TNF-, IL-6, IL-8, interferon (IFN)-, IL-12p40, IL-1 and IL-17) and enhanced apoptosis-regulating proteins (caspase-8, Bax), highlighting its potential for managing intestinal inflammation

Pathway analysis indicated that 47 of the 94 genes are involved in metabolic pathways such as ferroptosis, D-glutamine and D-glutamate metabolism, and autophagy (Figure 3C)

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