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Protein extracts were separated by electrophoresis on 412% NuPAGE Bis-Tris Mini Gel and then transferred onto nitrocellulose membranes using iBlot transfer stacks (Life Technologies, Gaithersburg, MD, USA)

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The chemical structure of glutathione contains three types of amino acids, among which glutamic acid and cysteine are in the L-type configuration, which is the common form of amino acids in proteins in nature

Retin Cases Brief Rep 10(3):232235 Ghosh S, Karin M (2002) Missing pieces in the NF-kappaB puzzle