Results S-palmitoylation maintains PM localization of GLUT1 To determine whether subcellular localization of GLUT1, the widely expressed glucose transporter responsible for the constant uptake of glucose 19 , is regulated by S-palmitoylation, we examined its cellular distribution upon treatment with 2-bromopalmitate (2-BP), a pan-inhibitor of PATs
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Briefly, 50100 l of saturated culture was spun down and cells were thoroughly resuspended in 100200 l of lysing buffer (62.5 mM Tris-HCl, pH6.8, 10% glycerol, 2% SDS, 4% -mercaptoethanol)
The Phase 2 trial (Jastreboff et al., NEJM 2023) tested doses from 0.5mg to 12mg
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