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Two different PCR reactions were performed, the first with 2 primers upstream and downstream of the first gRNA target (Fw primer 5-CCACTTTTGCTGCAGTTGCT-3 and Rw primer 5-TGCTGAGACATCGCTGATCC-3) and the second with the same forward primer and as reverse an oligonucleotide downstream of the second gRNA target (5-TGAAAAGGCCCCCATGTGAA-3)

doi: 10.1038/s41568-018-0007-6 22 ZhaoZChenXDowbajAMSljukicABratlieKLinLet al