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Remarkably, the animals later performed as well as rested mice in a memory task, despite having stayed awake

JAMA 331 , 10071008 (2024)

Proteins were separated by SDSPAGE, transferred to PVDF membranes (Millipore) and probed with primary antibodies in 1 TBST buffer (20 mM Tris pH 7.5, 150 mM NaCl and 0.1% Tween-20) with 5% fat-free milk at 4 C overnight

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The few exceptions include delayed coverage for some infusion medications considered for coverage under the TRICARE medical benefit

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