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The Dulbeccos Modified Eagle Medium (DMEM) (Gibco, 11995065) that was supplemented with 10% fetal bovine serum (FBS) (Cytiva, SH30071.03HI-LSF) and 1% streptomycin-penicillin (Gibco, 15140122) was used for cell lines received through Duke University

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TB-500 (thymosin 4) drives systemic cell migration and angiogenesis, accelerating endothelial migration several-fold in preclinical research[2]