Site-directed mutagenesis Plasmids encoding Flag-tagged MFAP2 wild-type (Flag-MFAP2-WT), His-tagged ITGB8 wild-type (His-ITGB8-WT), and His-tagged ITGB8 ARG57 mutant (His-ITGB8-ARG57) were transiently transfected into the cells using Lipofectamine 3000 (Thermo Fisher Scientific, Pittsburgh, PA, USA)
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There was also no significant difference found in the secondary outcome of general distribution of specific hangover symptoms including headache with NAC and Placebo both with median = 1 ( P = 0.93), nausea with median score for NAC = 0, and Placebo = 1 ( P = 0.11), and weakness with NAC median = 1, and Placebo median = 1 ( P = 0.72)
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SCOT, succinyl-CoA:3-oxoacid-CoA transferase