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The higher the intracellular concentration of GSH, the better the protection against oxidative stress, disease progression, and ageing

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+ lining macrophages limiting the inflammatory response by providing tight junction-mediated shielding for intra-articular structures (Kurowska-Stolarska and Alivernini, 2022

The direct reaction of these side chain functional groups results in macrocycle formation, with no introduction of additional linkers

Alternatively, cells were harvested with trypsinEDTA solution, suspended in culture medium and the cell number was determined using a Countess Automated Cell Counter (Life Technologies)

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